Which is the plant vacuolar dye for localization studies??

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In multi-label classification, why the sample-based performance evaluation metrics are usually lower than the label-based performance metrics?

Hi all, I am doing a multi-label classification using machine learning algorithms. In order to evaluate the performance of the multi-label classification, I am using label-based and sample-based...

13 January 2021 7,590 3 View

In multi-label classification, how do we find the P values and confidence interval for the performance evaluation metrics?

Hi all, I want to report the P values and confidence intervals for AUC values for two different multi-label classifiers. I got the following article, which is applicable for a binary...

10 January 2021 2,217 3 View

What are the approaches of improving SNR of spaceborne Hyperspectral instrument for Earth observation or Planetary science missions?

Spaceborne Hyperspectral observation (i.e. hyperspectral remote sensing in uv-visible-infrared spectral range) of Earth and for Planetary science, plays a very important role in improving...

27 December 2020 453 2 View

How to extract and store elemantal strain results data of a FEA using ansys in a text file for postprocessing?

I am doing a bone remodeling simulation using ANSYS. i need to extract elemental strain results and store in a text file for further processing. There is no option using *GET and *VGET command...

15 November 2020 2,731 2 View

How can I obtain Nodal Forces at selected nodes/elements for all time steps in a transient analysis in ANSYS?

I am working on a transient structural analysis in Ansys Mechanical. I want to export the Nodal reaction force vectors for selected nodes of a part at all the time steps. Basically, I need a force...

18 October 2020 9,789 3 View

Can we use gravity to travel one place to other ?

Just dig a deep hole perpendicular to the surface of earth. Later when we would be near core then may be repulsive forces of core may help us to move straight forward and come out to other part of...

12 March 2019 6,222 3 View

For contact stress analysis in gears, which stress in ANSYS represents contact stress, whether to use Von mises stress or contact pressure?

In ANSYS we get different stresses for gears under contact. So which stress should be used to compare with Hertzian contact stress.

10 March 2019 6,032 5 View

Which algorithm is used by commercial FEA solvers for Topology Optimization?

These commercial FEA solvers do Topology Optimization after FEM Simulation but on what basis they remove elements from the model.

02 January 2019 497 11 View

How cohesion parameter COHE effects the frictional stress in ansys for coulomb frictional module?

The frictional stress for coulomb frictional model is defined as sum of cohesion parameter and mu*contact pressure.

28 October 2018 2,535 2 View

How to get rid of contact chattering in ansys mapdl?

My simulation is not converging because of contact chattering value which i found is 1. I am using augmented Lagrangian method with FKN-1, PINB- 0.1, FKT-1, FTOLN- 1, RESULTING PENETRATION...

13 September 2018 8,088 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View