Which is the plant vacuolar dye for localization studies??

More Ankush Saddhe's questions See All

If my gene of interest has high GC content can it be problematic in sequencing? What kind of error is expected with GC rich gene sequences??

Gene sequencing related trouble shooting

25 July 2024 4,149 2 View

Can someone provide the detailed protocol for the synthesis of lanosterol nanoparticles?

There is no information present in the databases regarding the synthesis of lanosterol nanoparticles.

28 April 2024 2,079 3 View

Is 2D-DIC sufficient for assessing damage on carbon fiber-composites in compression after impact test?

We have a 2D DIC setup and want to know if there are any open source/commercial packages which can help us assessing the out of plane deformation due to compression of the coupon (carbon fiber...

21 November 2023 2,850 0 View

How to generate random pores within a CAD geometry with a desired porosity and pore sizes ?

I have a solid CAD model geometry (complex shape). I want to generate random spherical pores within that geometry with desired porosity and pores size.

11 May 2023 6,275 2 View

Is it normal for organoids to form clump in differentiation culture?

I am trying to make midbrain organoids from iPSC. After matrigel embedding, when I transferred organoids to ULA 6-well plate for differentiation. I found organoid-formed clumps in the media. Is it...

25 April 2023 7,739 1 View

How to use references in latex with standard templates?

I am a newbie to latex. I was trying to use references in latex. I was attempting to have the author's name and the year of publication appear in my literature section in place of reference...

26 January 2023 8,360 2 View

Latent Heat of Manganese Dioxide (MnO2) ?

I am looking for the heat of the fusion of manganese dioxide. I am unable to find it in the literature.

11 January 2023 5,812 4 View

Which cruicble can be used for TG-DTA of NaOH(s) samples ?

I want to perform TG-DTA on NaOH in an inert medium. Generally, alumina and platinum crucible are used for TG-DTA. However, I am confused with respect to crucible material compatibility with NaOH...

12 December 2022 6,754 2 View

What are the mega trends and recent debates in SPIONs Contrast Agents for MRI?

SPIONs has been extensively explored as the Contrast Agent for MRI. What are the current mega trends that are in this field? What are the challenges that are still to be achieved and overcome?

05 September 2022 7,207 4 View

Most contagious and contaminat dyes for study in Photocatalytic dye degradation?

Hello RG experts I am working on dye degradation and looking for dyes which are suitable for todays scenario. Could you all please enlist some of the most contaminant dyes that are suitable for...

28 January 2022 1,331 7 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...

29 July 2024 2,682 6 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Is it necessary to attach fluorophore at 3' end in MIC RT PCR machine for Dual hybridization Probe designed especially for genotyping assay?

I designed 2 dual hybridization probes for my single SNP. where I attach my fluorophore 5'-FAM-nnnn-BHQ1-3', and 5'-HEX-nnnnnn-BHQ2-3'. I need to run it on the MIC RT PCR machine by Biomolecular...

28 July 2024 5,658 0 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Problems with ion exchange chromatography?

I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...

27 July 2024 6,605 0 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View