Which is the most compatible solvent for the extraction of neutral lipids mainly the TAG ?

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What are the journal lavalible for the research area in deep learning and domain breast cancer?

suggest any list of journals to publish my article with impact factor 3-4 impact factor

23 July 2024 6,219 2 View

How can I calssify samples in multiclass EEG Motor Imagery dataset in MATLAB?

1) In EEG Motor Imagery multiclass dataset, e.g., BCI Competition IV dataset IIa, which is a 4-class dataset (Right Hand, Left Hand, Tongue and both Feet). How can I classify the samples with...

14 June 2024 3,570 1 View

Is there a way to estimate the covariance of a censored variable with a continuous variable in Mplus?

I am estimating the transactional associations of two LGC processes (maternal depressive symptoms and child externalizing behaviors) and would like to include the concurrent covariances of their...

29 May 2024 9,800 0 View

How do I perform data analysis in Excel on two variables with varying amount of questions and values?

I am studying leadership style's impact on job satisfaction. in the data collection instrument, there are 13 questions on leadership style divided into a couple of leadership styles. on the other...

16 April 2024 4,784 3 View

Hi! Please suggest the appropriate tests for hypothesis testing?

My Phd is in HR area. I want to study the impact of independent variable on dependent variable. And study whether there is significant positive relationship between IV & DV. in such...

24 March 2024 1,932 4 View

Why am I continuously getting positive signal in my 3 plex negative control in the RNAscope multiplex V2 kit?

I am currently working to optimise RNAscope multiplex V2. I have troubleshooted the issue with several tests. I have washed thoroughly with wash buffer even with their recommended guidelines of...

29 February 2024 4,764 0 View

Self-consistent clustering analysis method for concurrent multiscale simulation?

Hi everyone, I want to ask how to use self-consistent clustering analysis method for concurrent multiscale simulations and how to solve Lippmann-Schwinger equation for this.

04 January 2024 2,601 3 View

How can I improve the DNA yield of my sample (hermit crabs)?

I am using the High Pure PCR Template Preparation Kit (Roche) to extract DNA from hermit crabs. These samples are stored in 99% ethanol at room temperature. I have found that many of them exhibit...

28 November 2023 3,989 1 View

Which M and SD should I use for calculating Z-score for a pre-post test RCT design?

I have two datasets (both with experimental group and control group) which measure the same construct with two different forms due to age differences. All groups completed the measure at a pretest...

08 November 2023 6,667 1 View

Why did not get transgenic plants even followed the existing protocol?

We have numerous previous articles and specific protocols by which you can regenerate transgenic rice plants. However, following the existing methods/protocol, if you do not get the positive...

01 November 2023 9,301 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

How to dispose off lipids waste?

I am looking for a lipid waste disposal method, keeping in mind the environmental, health and safety aspect of lipid waste. Could someone please provide guidelines or the lowest acceptable...

25 July 2024 637 5 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View