I wish to see the expression changes of both miRNA and gene expression in blood lymphocytes of subjects (before and after treatment).
I wonder which is is the best way to calculate the the sample size? what parameter i need to consider?
I request for suggestions
Thank you
Venky
I did notice that google has links to at least one sample size calculator, which I have not used. If you want to try it yourself, so that you can keep track of the calculation on your own, try the the following link.
https://en.wikipedia.org/wiki/Sample_size_determination
I used formulas similar to those shown by Wikipedia some time ago. The formulas must have come from a statistics book such as the ones by Steel and Torrie and by Snedecor and Cocheran (spelling) as Wikipedia was not yet available.
Michael
Dear Venky Raavi, there is a particular good R package available for this purpose called RNASeqPower. Please see:
https://www.bioconductor.org/packages/release/bioc/vignettes/RNASeqPower/inst/doc/samplesize.pdf
There is also a web based alternative designed for this purpose:
http://scotty.genetics.utah.edu/
However, i both cases you would need to do some preliminary experiments to determine the varriance in your NGS datasets. Based on that you will be able to estimate roughly the required sample size.
However, the sad reality is often that you have financial limit on how many samples you can sequence and that number (depending on your financial situation) is often well below that number suggested by a power analysis. Nevertheless it is a good starting. I hope this helps.
All the best,
Hannes
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