We have some herbs and we have been planning for hyroalcoholic extract from the herb. After extraction we have a plan for isolation and identification of bioactive plant metabolites.
Need your kind suggestion.
I think that, the type of herbs could influence the bioactive metabolites in a particular hydroéthanolic ratio extraction. so, if you are targeting a specific group of métabolites according to the biological activities that you are searching for; first make a rapid dosage of these groups of metabolites in differents hydrohethanolic ratio (50:50; 75:25; 80:20.....); then you will choose the best ratio and continue your isolation
70% hydroethanol with 0.3% citric acid solution. 3 times. The organic solvent was removed under vacuum. And the resulting crude was futher freeze dryed.
You can start from increasing order of hydrophilic solvent ( Methanol> Ethanol>Acetone.... ) or various ratio in combination for evaluation of plant extracts as follows:-
A. 50% Methanol (1:1) ratio of water and Methanol (hydrophilic compounds)
B. 50% Ethanol (1:1) ratio of water and Ethanol ( it is used for less hydrophilic compound)
You can try aqueous ethanol and then varying the ethanol concentrations. By doing so, you will be able to arrive at the best percentage of ethanol for extracting each plant.
Best solvent ratio of Water and Alcohol is varies with herbal material or part like Flowers, Leaves, Roots, Stem bark, Seed etc. Whether it is fresh or Dry? Whole plant etc. In general ratio is taken 50:50 of each.
Prof Gandhidas Sonajirao Lavekar
Thank you for your suggestion.
Its actually dried unripened fruits.
I think that, the type of herbs could influence the bioactive metabolites in a particular hydroéthanolic ratio extraction. so, if you are targeting a specific group of métabolites according to the biological activities that you are searching for; first make a rapid dosage of these groups of metabolites in differents hydrohethanolic ratio (50:50; 75:25; 80:20.....); then you will choose the best ratio and continue your isolation
F. Romain Fouelefack
Thank you for your suggestion. Would you please give me any articles regarding your suggestion?
Dear A S M Ali Reza,
They are a lot of biological Activities (Antifungal, Antibacterial, antioxydant, Anticancer…., you may be interested in !!
For exemple; it is known that many flavonoids have antioxydant activities. So if the aim of your work is to isolate compounds with antioxydant properties from hydroalccolic extracts of your plants, you may rapidly quantify the flavonoid (Articles attached) content of different hydroalcoolic extraction ratio ((0:100; 50:50; 75:25; 80:20.... and then choose the ratio that exhibit the hight flavonoids content You could then after try to isolate flavonoid compounds using different chromatographic methods.
If you are interested in alkaloids which are, stimulants, narcotics, poisons you may first quantify alkaloids as described in article “primary and second metabolite quantification++++A11” attached below
You could also use phytochemichal screening (Articles attached) but the precision is less
NB:The extraction procedure with different hydroalcolic ratio, should be caried out in about the same experimental condition, to avoid the influence of other factors.
See as attached files some Articles regarding the these sugestions, hoping that they will help in your work
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