It is not possible to predict the best solvent or extraction method for determining antidiabetic activity without first conducting a pilot study. It is recommended to test various solvents with different extraction methods to analyze the antidiabetic activity. Only after conducting these tests can you determine which solvent and extraction method are most suitable then only your research further study will conduct. The activity is primarily influenced by bioactive compounds, which possess unique polar and non-polar properties. Therefore, conducting the tests in different solvents and extraction methods is essential, and the results may vary depending on the plant sample used.

In general

• Extraction methods such as maceration, soxhlet, and ultrasonic extractions are relevant for isolating bioactive compounds from plant materials which exhibit antidiabetic properties including those that inhibit alpha glucosidase.
• The solvents like ethanol,methanol,water,acetone and hexanes are effective in extracting phytochemicals that have shown potential antidiabetic effects, including flavonoids and phenolic compounds.
• As per your concern, try using 70% ethanol and 30% water mixture for extraction as it balances polar and moderately non-polar compound extraction effectively and as of extraction method try Ultrasonic-assisted extraction this will further enhance yield and efficiency.
• Based on your specific plant material maintain optimal extraction conditions

Nesibe Arslan Burnaz

Majority of plant based amylase and glucosidase inhibitors are actually the polyphenolic compounds, including phenolic acid and flavonoids. For an example, quercetin showed a significant enzyme inhibitory activity as equipotent as standard acarbose and voglibose. Based on that, I will suggest you to go with 70% MeOH for extraction, which will lead to partition of all water soluble phenolic acids and polar secondary metabolites.