My application for ethics was deferred because the reviewers said that administering Doxorubucin via an i.p injection will cause blistering and that it should be given i.v, however, every paper that I have read administers Doxorubicin i.p. I was just wondering if anyone has any information/articles that can substantiate and motivate why I am giving the injection i.p. All the papers I had read give the injections intraperitoneally, however, they do not state whether there were any negative effects, and this is what concerns the ethics committee. Are there advantages and disadvantages to given the drug iv vs ip? Thank you!
IV infusion of dox would bring with it lot of side-effects due its potency as cytotoxic agent; and you are directly sending it into blood stream-extremely detrimental, hence IP is slightly better. You want to comment about the tolerable dosage as well.
I think people give it i.p. due to ease of administration. If the drug is soluble in aqueous vehicle you can use i.v. route and probably will lower dose than i.p.
Dear Toni,
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2004 Dec;148(2):161-6.
Pharmacokinetics and biodistribution studies of Doxorubicin loaded poly(butyl cyanoacrylate) nanoparticles synthesized by two different techniques.
Reddy LH, Murthy RS.
The aim of the study is to determine and compare the pharmacokinetics and tissue distribution of Doxorubicin (Dox) delivered as solution or through nanoparticles after intravenous (i.v.) and intraperitoneal (i.p.) injection.
Thank you so much, everyone! Really appreciate your responses!
Hi colleagues,
My coworkers had the same issue with ethical comity and they performed a pilot study to optimize Doxo dose in C57BL mice for inducing cardiotoxicity.
They found IP injection of Doxo has a strong toxic effect on intestine with big dystrophic changes, pain, etc.
The best results obtained from the tail vein injection. No animal loss registered for Doxo dose of 4 mg/kg and 20 mg/kg at endpoint (7-8 days) after injection in both sexes.
But the most important finding was to control water availability since animals were very weak after Doxo injection and sometimes not able to use central water supply system or bottles. So put transportation gel or just food pellets soaked with water available for animals and it will improve survival rate a lot.
Hi Daniil,
We need to give Doxo iv but have a criticism from our committee about local toxic effects etc. Do you know if your co-workers have published their pilot study or would be prepared to share their data?
Andrew
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