Which is the best promoter to use to make transgenic mice with stem cell specific expression?

More Subbarao Bondada's questions See All

Any old or new technology for artificial rain making other than cloud seeding?

Rainfall is the major source for water and soil moisture in the semi-arid and dry locations. During summer monsoon season due to failure of rains, agriculture becomes vulnerable and crops faces...

20 August 2021 5,293 5 View

How Nanotechnology helps to develop various products in Agrometeorology?

Nanotechnology (NT) is one of the advanced material science with which the development in materials and improvement in electronics. NT has made it possible to miniaturizing the things to fit in...

21 January 2021 2,699 6 View

Is there any pre designed Deep networks are available for signal classification like googlenet and alexnet?

Signal classification using Deep Learning

24 April 2019 4,689 3 View

Are there any reporter assays for functionality test of miRNAs targeting in the coding region or the 5'UTR regions of the respective mRNA target??

I am looking for luciferase reporter-like assays for proving the functionality of miRNA targeting the 5'UTR or the coding regions in the mRNA. Kindly, suggest me if there any assays established in...

10 May 2018 230 3 View

Can I isolate mitochondria using a bench centrifuge at room temperature?

Hi all, I am wanting to isolate mitochondria from BMDMs infected with mycobacterium tuberculosis - so this needs to be done in CL3 conditions. We have a benchtop microfuge which can only run at...

27 September 2016 8,647 10 View

Best FFT window for Modal analysis?

I am exciting a piezo-ceramic actuator by sine wave (particular frequency) and recording velocity information from laser vibrometer. I am using rectangle window for my analysis so far. I came...

02 December 2015 1,432 6 View

What are some interesting EEG experiments that require no/minimal postprocessing and can be subjectively correlated with one's mental state?

What I'm looking for are nontrivial (e.g. something more interesting than eyeblinks) experiments where the correlations between objective data and subjective experience are clearly distinguishable...

22 May 2014 6,135 2 View

What is the best EEG hardware and software currently available that an independent researcher can purchase?

Hardware -- preferably greater than 15 channels, looking for the least noisy EEG signal. Also looking for recommendations on the best quality dry sensors. Software -- looking for packages that...

22 May 2014 8,161 5 View

How Agrometeorology can help in mitigating or adaption of crops in variable weather conditions of the future?

Agrometeorology is broadly explained as the subject of weather / climate in relation to crop and its sustainability. It encompasses climate, micrometeorology, soils, water, plant , pest / diseases...

01 January 1970 8,183 37 View

How can we use Artificial Intelligence, Machine Learning and deep learning tools along with IOT in Agricultural Meteorology?

Computer applications have gone to a next level with the advent of AI, ML and DL applied to any kind of subject, analysis, simulation, automation and control. IOT is another handy technology for...

01 January 1970 5,290 13 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

What are those vacuolated structures in my tilapia intestinal cross-sections (H&E stained)? Do the sections exhibit signs of enteritis?

Could someone please confirm if those vacuolated structures are goblet cells? And whether the sections exhibit signs of inflammation. Context: I am conducting histological analysis of the...

15 June 2024 3,208 4 View

Is there anything called cd 12 in immunology?

define cd

06 June 2024 9,533 5 View

How can I put an alert to receive notifications of call for papers in my research field?

I'd like to receive notifications of call for papers for any journal pertaining to my expertise (immunology, rheumatology, immuno-mediated liver involvement) from researchgate. Thanks in advance...

01 June 2024 1,352 0 View

How many percent of specific antibodies in serum after rabbit immunization ?

Hi all, I'm trying to validate an ELISA against anti-drug antibodies in which I use a immunized rabbit serum as positive control. Rabbit was a NZR, immunization protocol was "standard" but I...

13 May 2024 2,828 0 View

What role do autoimmune disorders play in causing infertility?

how can immunological interventions potentially address these issues?

04 April 2024 4,951 1 View

PhD candidate with a research focus on chronic inflammation particularly macrophage cells so i need professor information this area??

I am a PhD candidate in immunology at AWKUM with a research focus on chronic inflammation, particularly the role of macrophage cells. I am writing to express my keen interest in your research on...

28 March 2024 7,798 0 View

How can I add weight to difference in correlation between 2 groups to also reflect the higher correlation values in addition to the difference?

I have several pairs of parameters (obtained from females and males) and want to find the difference in correlation between the two sexes for each parameter, but also want to give a weight so that...

11 March 2024 1,000 4 View

Daniel Clark

Are you trying to express a certain gene, or protein, specifically from the murine stem cells? Is your experiment in vivo, or ex vivo?

If you clarify your hypothesis and protocol you will have more chance of getting answers to your question!

Subbarao Bondada

I never came back to this site since I posted this question.

We want to express a gene in vivo. Our experiment will be in vivo.