Hi, I would like to work on antimicrobial properties of parsley on UTI in vivo. I found different methods talking about drying grinding and making parsley capsules. Some talk about ethanol extraction. To test antimicrobial effect in vivo on UTI which is the best? and please with references.
Regards
Hi,
Extraction method not a limit for AMA.
but of course play an important role on molecules which has AMA.
here, first, both polar and and non polar molecules have the AMA. so select the solvent based on its polarity of the molecules you want to test.
Second, if searching new molecule use solvents like methanol but the traces should be removed before invivo. so that people suggesting Ethanol but ethanol not have domination on all kindly of molecule.
Third, hot extraction may degrade desired molecule.
So, better to try, cold or low temperature. if some experts suggesting water then try free drying after extraction.
but drying the leaves and making capsule and trying Invivo is not advisable because it very crude method and take long way to achieve the right molecule.
My answer may not so conclusive. but may help with some idea.
All the best
This is research you can try different types of extraction available in your lab. Most time its better to perform your own experiments rather than relying too much on literatures giving one method better than the other. The operating condition, geographical location where u harvested your parsly and the equipment model has great influence on your result.
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