The alga is a marine one hence the medium suggested could be neutral with regards to nitrogen experiment..
I agree with Sarah: Bold 3N (without nitrate) as described by Berges: Berges, J.A., Franklin, D.J., Harrison, P.J., 2001. Evolution of an artificial seawater medium: improvements in enriched seawater, artificial water over the last two decades. J. Phycol. 37, 1138–1145.
Although it may seem to be more suitable for cyanobacteria, we (and many our colleagues) did it successfully with BG11 lacking nitrate (see Rippka R., Deruelles J., Waterbury J.B., Herdman M., Stanier R.Y. Generic assignments, strain histories and properties of pure cultures of cyanobacteria // J. Gen. Microbiol. 1979. V. 111. P. 1-61).
It being a marine algae I would recommend making a simple F/2 media without adding nitrogen. Its simple to make and you can, if you choose to, add nitrate or another N source later should you want to confirm limitation. Also I generally define limitation when the growth rate is about half of that in the replete culture. I have also used GSe media of much of my culture work. IF you need recipes I can supply them.
Thank you very much Florian Koch for your suggestions. Infact i have started with f/2 medium. kindly send me the other recipes too. Thanks a lot!
Thanks
Alexei Solovchenko, Gerd Kloeck and Sarah Heath! i had tried with BG-11 medium. But the results were very random and i could not follow them. Now i have started afresh with f/2 medium.
Important question you have to ask yourself is to what biomass concentration you expect your culture to grow. When this is high, you need to use a concentrated medium, otherwise you run the risk of running out of other nutrients (for instance P) halfway during your N-starvation experiment.
For 'dense' cultures, we routinely use the media described in attached paper. Seems to work fine for virtually all algae we cultivate in our lab. They are designed to support a dry weight biomass increase of around 5 gram per liter, above which nutrients will become depleted (which nutrients depends on the elemental composition of the microalgae of course). Note that there is not always a need for the vitamins.
Hope this helps, best regards,
Packo
Article The impact of nitrogen starvation on the dynamics of triacyl...
BTW, we are organising a course on Microalgae Biotechnology that might be of interest to you:
Microalgae Process Design - from cells to photobioreactors. July 10 - 17, 2015, AlgaePARC, Wageningen University, the Netherlands.
This course provides the essential skills for designing optimal microalgae-based production processes, for both research and commercial purposes.
Through lectures, digital cases and a photobioreactor practical, the participants will learn: 1) how to describe microalgal metabolism quantitatively, 2) how to apply basic design principles and set up mass/energy balances for photobioreactors, 3) how to cultivate microalgae in fully controlled photobioreactors, and 4) how to integrate all acquired knowledge into optimal production strategies for microalgae biomass or secondary metabolites.
Previous editions were rated at 9.2 (2013) and 9.8 (2014) out of 10 by course participants.
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Hi Packo, thank you for your sharing. I am interested in your biotechnology course. Would you give me or information so as to attend it, thanks.
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