Hi scientists,
i have some questions for you. I'm trying to performe fEPSP experiments to induce LTP in hippocampal CA1, but i have some problems finding a good response. I would to record fEPSPs from the dendritic zone of the CA1. I'm thinking that one of the problems could be the slicing angle. Which is the best angle to cut healthy coronal hippocampal slices? Another question is about the stimulating electrode. I usually use a glass pipette filled with acsf to stimulate the fibers . Is a good choice or i could use best stimulating electrodes?
Thanks so much for help ;)