10 Questions 8 Answers 0 Followers
Questions related from Kevin C Chen
In the trafficking studies of active /inactive integrins, scientists usually employ conformation-specific antibodies (e.g., mAB13 for inactive integrin beta 1; HUTS-21 or 9EG7 for active beta 1)...
15 July 2022 6,696 0 View
When I need to assay the expresssion of a certain membrane-bound protein on cell surface, I usually perform Western blot of the membrane proteins extracted by detergents such as NP40 or Brij,...
16 May 2022 8,496 1 View
Hi, I am using ion-selective microelectrode to measure the extracellular concentration of a certain ion probe (in this case, tetramethyl ammonium, TMA+). The ISM is double-barreled, with one...
30 March 2018 9,953 0 View
I am recently switching to WPI Duo-773 to measure extracellular diffusion of tetramethyl ammonium (TMA+, a small cation commonly used to measure the brain extracellular space) by the real-time...
06 March 2017 7,424 1 View
Hi,my question is about the tip potential often associated with the use of glass microelectrodes. I read the book of "Ion-Selective Microelectrodes" by Daniel Admmann (a classic textbook for...
24 July 2016 5,663 2 View
I want to compile a list of acute pathological or physiological events that last no more than 5 min. Currently, I can only think of cortical spreading depression (CSD) and seizure that qualify for...
15 December 2015 1,802 6 View
I want to do fEPSP recordings on the Shaffer-CA1 pathway in vivo in the middle part of hippocampus in rat. I checked the literature and found conflicting results. For example, in the work of Doyle...
25 November 2015 9,196 4 View
I am working on a mass diffusion problem within a porous medium with time- and space-varying porosity (epsilon) and apparent diffusion coefficient (D*). That is, epsilon and D* are both fn(x, y,...
07 September 2014 7,590 4 View
During the firing of an action potential (AP) K+ ion outward flux causes membrane potential repolarization, even hyperpolarization. But according to the Goldman-Hodgkin-Katz equation, increasing...
18 March 2014 6,132 22 View
I know that fasting leads to increased level of intracellular NAD and activation of SIRT1. But does anyone know the underlying mechanism relating fasting to NAD?
21 June 2013 3,359 2 View
