Kevin C Chen

6 Questions 5 Answers 0 Followers

Questions related from Kevin C Chen

Would conformation-specific integrin antibodies fail to track integrins when integrin conformation changes?

In the trafficking studies of active /inactive integrins, scientists usually employ conformation-specific antibodies (e.g., mAB13 for inactive integrin beta 1; HUTS-21 or 9EG7 for active beta 1)...

15 July 2022 6,665 0 View

Which method of membrane protein extraction is better?

When I need to assay the expresssion of a certain membrane-bound protein on cell surface, I usually perform Western blot of the membrane proteins extracted by detergents such as NP40 or Brij,...

16 May 2022 8,455 1 View

How to get the right response from an ion-selective microelectrode?

I am recently switching to WPI Duo-773 to measure extracellular diffusion of tetramethyl ammonium (TMA+, a small cation commonly used to measure the brain extracellular space) by the real-time...

06 March 2017 7,390 1 View

Does an ion-selective electrode have a tip potential?

Hi,my question is about the tip potential often associated with the use of glass microelectrodes. I read the book of "Ion-Selective Microelectrodes" by Daniel Admmann (a classic textbook for...

24 July 2016 5,632 2 View

Any examples of acute pathological events other than spreading depression & seizure?

I want to compile a list of acute pathological or physiological events that last no more than 5 min. Currently, I can only think of cortical spreading depression (CSD) and seizure that qualify for...

15 December 2015 1,777 6 View

How do I place stimulating & recording electrodes for in vivo hippocampal fEPSP recordings?

I want to do fEPSP recordings on the Shaffer-CA1 pathway in vivo in the middle part of hippocampus in rat. I checked the literature and found conflicting results. For example, in the work of Doyle...

25 November 2015 9,168 4 View