8 Questions 29 Answers 0 Followers
Questions related from Luca Murru
Dear colleagues, I'm performing LTD experiments in the mouse hippocampus (CA1) using coronal brain slices and recording fEPSP. Mice are 30 days old and I'm trying low frequency stimulation (900...
19 December 2022 9,502 2 View
Dear collegues, I have some electrophysiological data about alterations in voltage-gated potassium channels mediated currents in a mouse model of neurodevelopmental disease and, considering the...
05 September 2019 2,144 0 View
Hi collegues, I'm trying to study the glutamate release probability in hippocampal cultured neurons. I started using hyperosmotic extracellular solution (1M sucrose) but this method evaluates the...
06 February 2017 286 10 View
Hi everyone, I'm trying to induce chemical LTD at the CA3-CA1 hippocampal synapse using DHPG 100 uM as reported in literature but at the moment i'm not able to induce a stable LTD after 10 minutes...
25 May 2016 852 13 View
Hi guys, I'm a little bit in trouble with LTP on CA1. I performed some experiments inducing LTP after HFS (1 for 100 stimuli at 250Hz). This protocol is able to induce a strong potentiation of the...
14 March 2016 441 6 View
Hi people, I performed some experiments in Ca1 pyramidal neurons to study sodium and potassium currents. I used classic I/V recordings (10 mV steps starting from -80mV with the cell clamped to...
11 February 2016 7,519 6 View
Hi all, I'm in troubles with some LTP experiments in those days. I'm trying to perform some experiments for a paper revision but i cannot induce LTP. Obviously before submitting the paper i've...
04 December 2015 414 13 View
Hi scientists, i have some questions for you. I'm trying to performe fEPSP experiments to induce LTP in hippocampal CA1, but i have some problems finding a good response. I would to record fEPSPs...
03 December 2015 8,330 3 View