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Questions related from Luca Murru
Dear colleagues, I'm performing LTD experiments in the mouse hippocampus (CA1) using coronal brain slices and recording fEPSP. Mice are 30 days old and I'm trying low frequency stimulation (900...
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Dear collegues, I have some electrophysiological data about alterations in voltage-gated potassium channels mediated currents in a mouse model of neurodevelopmental disease and, considering the...
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Hi collegues, I'm trying to study the glutamate release probability in hippocampal cultured neurons. I started using hyperosmotic extracellular solution (1M sucrose) but this method evaluates the...
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Hi everyone, I'm trying to induce chemical LTD at the CA3-CA1 hippocampal synapse using DHPG 100 uM as reported in literature but at the moment i'm not able to induce a stable LTD after 10 minutes...
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Hi guys, I'm a little bit in trouble with LTP on CA1. I performed some experiments inducing LTP after HFS (1 for 100 stimuli at 250Hz). This protocol is able to induce a strong potentiation of the...
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Hi people, I performed some experiments in Ca1 pyramidal neurons to study sodium and potassium currents. I used classic I/V recordings (10 mV steps starting from -80mV with the cell clamped to...
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Hi all, I'm in troubles with some LTP experiments in those days. I'm trying to perform some experiments for a paper revision but i cannot induce LTP. Obviously before submitting the paper i've...
04 December 2015 415 13 View
Hi scientists, i have some questions for you. I'm trying to performe fEPSP experiments to induce LTP in hippocampal CA1, but i have some problems finding a good response. I would to record fEPSPs...
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