Luca Murru

9 Questions 31 Answers 0 Followers

Questions related from Luca Murru

Any tip for LTD induction in CA1?

Dear colleagues, I'm performing LTD experiments in the mouse hippocampus (CA1) using coronal brain slices and recording fEPSP. Mice are 30 days old and I'm trying low frequency stimulation (900...

19 December 2022 9,554 2 View

Do you have any suggestions about a pan antibody for voltage-gated potassium channels?

Dear collegues, I have some electrophysiological data about alterations in voltage-gated potassium channels mediated currents in a mouse model of neurodevelopmental disease and, considering the...

05 September 2019 2,191 0 View

How can I study plasma membrane composition and structure?

Dear collegues, I have a technical question about cell membrane study. I'm working on a transmembrane protein that belong to tetraspanins superfamily and I know that tetraspanins directly interact...

22 July 2019 8,507 4 View

How can i study glutamate release probability in hippocampal culture without using double patch recordings?

Hi collegues, I'm trying to study the glutamate release probability in hippocampal cultured neurons. I started using hyperosmotic extracellular solution (1M sucrose) but this method evaluates the...

06 February 2017 344 10 View

Why i'm not able to induce a stable DHPG mediated LTD in my preparation?

Hi everyone, I'm trying to induce chemical LTD at the CA3-CA1 hippocampal synapse using DHPG 100 uM as reported in literature but at the moment i'm not able to induce a stable LTD after 10 minutes...

25 May 2016 903 13 View

Why LTP in CA1 increases after the induction?

Hi guys, I'm a little bit in trouble with LTP on CA1. I performed some experiments inducing LTP after HFS (1 for 100 stimuli at 250Hz). This protocol is able to induce a strong potentiation of the...

14 March 2016 498 6 View

How can i analyse sodium currents from I-V relationship?

Hi people, I performed some experiments in Ca1 pyramidal neurons to study sodium and potassium currents. I used classic I/V recordings (10 mV steps starting from -80mV with the cell clamped to...

11 February 2016 7,568 6 View

What do you think about animal facility, stress and LTP induction?

Hi all, I'm in troubles with some LTP experiments in those days. I'm trying to perform some experiments for a paper revision but i cannot induce LTP. Obviously before submitting the paper i've...

04 December 2015 466 13 View

Which is the best blade inclination to cut very healthy coronal hippocampal slices?

Hi scientists, i have some questions for you. I'm trying to performe fEPSP experiments to induce LTP in hippocampal CA1, but i have some problems finding a good response. I would to record fEPSPs...

03 December 2015 8,388 3 View