HI everybody,

Is there anyone who had the venture to try an immunohostochemical reaction on formalin-fixed, sucrose-cryoprotected, OCT embedded cryosection with anti-ki67 antibody?

The reaction does not work either straight or upon retrieving antigenicity with citrate buffer at 98° for 1 hour. Is there any antigen-retrieval technique that is known to work in such conditions?

All the best

Eugenio

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