HI everybody,
Is there anyone who had the venture to try an immunohostochemical reaction on formalin-fixed, sucrose-cryoprotected, OCT embedded cryosection with anti-ki67 antibody?
The reaction does not work either straight or upon retrieving antigenicity with citrate buffer at 98° for 1 hour. Is there any antigen-retrieval technique that is known to work in such conditions?
All the best
Eugenio