21 March 2022 5 2K Report

What are recommended solvent for gel and cream?

How much volume and concentration of solvent and gel/cream is required for MTT assay?

More Muthuraman M's questions See All
Is there any cases algae not using the nutrient from the wastewater and grow normally?

I am working on microalgae cultivation using waste water. The initial concentration of nutrients were less but the microalgae has achieved biomass growth of 2 g/L. The final concentration of...

08 August 2024 4,812 2 View

I have calcined my catalysts without flowing air from the compressed air cylinder will there be any problem with my reaction if i use that catalysts?

FYI Catalyst is MFI zeolite Si/Al = 140,25.40.11.5,15 I am using this catalyst to study methanol to DME conversion

25 July 2024 2,486 2 View

How can I measure arsenic concentration in a patient blood?

I intend to get an economical and accurate LAB TEST to measure arsenic concentration in blood sample.

23 July 2024 3,654 2 View

It is possible to conduct a structured written interview for a qualitative study?

In your opinion, I would like to know if a qualitative study with a structured interview can be conducted in writing. In other words, we sent out open questions and asked the interviewees to...

11 July 2024 4,163 4 View

What is Differential-Fed in microstrip patch ant? How it is design in CST studio? What is process and procedure to do Differential-Fed in CST studio?

What is Differential-Fed in microstrip patch antenna ? How it is design in CST studio? What is simulation process and procedure to do Differential-Fed in CST studio ?

08 July 2024 7,464 1 View

How to know the TM/TE modes in antenna design in CST studio?

How to know the TM/TE modes in antenna design in CST studio? Example: TM01 ,TM02 ,TM10, TM12, TM30 like this... how can identify the antenna is operate the above said mode? can any one explain...

08 July 2024 6,609 2 View

What is co-polarization and cross polarization? How can plot it from CST studio?

what is co-polarization and cross polarization? How can plot it from CST studio? what are steps and process for find out co-polarization and cross polarization from CST studio?

08 July 2024 7,869 1 View

Why in some degradation experiment the results doesn't fit first or second order kinetics ?

In an antibiotic degradation experiment using metals carbon composites to activate PMS, the results doesn't fit pseudo first or second order kinetics however the degradation efficiency is very...

26 June 2024 6,941 3 View

Why carrier Concentration decreases while increasing Temperature ?

Hi All . Recently, I conducted Hall measurement experiments at high temperatures on highly p-doped diamond with a doping concentration of 2.5×10^20 cm−3 During these experiments, I observed two...

23 June 2024 4,763 0 View

What are the best practices for increasing earth biomass?

I'm thinking of how to increase biomass to replace plastic and industrial waste.

23 June 2024 1,871 4 View

Similar questions and discussions
Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

What is the solvent for 8YSZ nano material?

I need to do some experiment with it in liquid form.

07 August 2024 613 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close