What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

How to export ZEN 3D time series?

I want to export 3D time series movie from ZEN. I found that I can only export one 3D image with its X or Y rotation instead of 3D-time series? I tried to use Fiji with the function of Z...

07 February 2021 5,365 1 View

(Scale request) Anyone has the Malay version of Eating Attitude Test-26/40 (EAT-26/40)?

Dear Researchers, My research team is conducting a eating disorder related research in Malaysia and we would like to use the Malay translated version of EAT26/40. I have tried to email the...

24 January 2021 8,925 2 View

Difficult to silence gene?

I have been trying to silence a gene of interest but I could not get silencing efficiencies beyond 50% My method of detection is via RT-qPCR and samples were extracted 48hrs post transfection. Are...

09 January 2021 3,404 4 View

HPLC separation for methyl salicylate, camphor, thymol and menthol?

I have been recently trying to develop a method to separate the above compounds. Most methods tried were adapted from literature using RP C8 or C18 column with ACN/MeOH and buffered water at pH 2...

28 December 2020 4,426 6 View

How to extract the different components at a specific frequency between two spectra?

Dear everyone: Hello! Sorry that my question may take up your valuable time. Recently I have a technical problem in the data processing. We have conducted a series of wind tunnel tests in order...

18 December 2020 8,847 2 View

There are prominent crystal formation in my cell culture dish, what are the possible causes and how to get rid of them?

17 December 2020 3,093 4 View

Has anyone extracted plasmid DNA from the Dmp1-GFPtopaz plasmid?

I just bought a plasmid from addgene, the name is DMP1-GFPtopaz. However, I could not extract the vector DNA by using Qiagen DNA purification kits from the bacteria. the bacteria growth was very...

05 November 2020 2,458 3 View

What corporate social responsibilities do social media companies have in relation to the use of psychographic profiling in advertising ?

What are the responsibilities of CSR ? What social media companies have done to protect the privacy or information of their users ?

12 October 2020 433 5 View

Testing significant differences between proportions when subjects overlap?

I am a bit stuck with this. Say for example there is a species of bird with variable colours, and they can present as red, blue, green or a combination (red-blue, red-green, blue-green,...

01 October 2020 3,761 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View