Fu Shun Wong

8 Questions 5 Answers 0 Followers

Questions related from Fu Shun Wong

How to ensure that the tumour is implanted at the mammary fat pad?

I am going to inject breast cancer (MDA-MB231) cells into female balb/c nude mice. I am injecting at the mammary fat pad. How would we ensure that the tumour is growing within or in the mammary...

25 November 2016 2,520 1 View

Why does intensity drops as concentration increases?

I am currently doing a fluorescence intensity scan with my compound. I am using the following parameters: Excitation: 273-276nm Slit: 20 Smoothing: 40 As my concentration increases from 20ug/ml...

17 November 2016 3,137 3 View

Can we use clean mice plasma for pharmacokinetic calibration curve?

I would like to know if there is any publication that supports the use of clean mouse plasma (eg. from sigma) to use it as a support for the calibration curve when compared to the PK of a mice...

05 October 2016 8,916 0 View

Is there any significant difference in pharmacokinetic profile between a tumour model and a healthy model?

I am studying the pharmacokinetic profile of vitamins in an in vivo model (mice). Correct me if I am wrong, for pharmacokinetic, I will be quantifying the concentration of vitamin E in the mice...

25 August 2016 3,403 5 View

Does anyone know whether it is suitable to use Alexa Flour Dyes for In Vivo Imaging?

I want to study the distribution of my nanoparticles in vivo, using a fluorescence tomography imager. I would like to know whether using Alexa Flour 488 dye or Alexa Flour 594 dye is suitable for...

15 August 2016 312 1 View

Which is better? Using EDTA or heparin blood tube?

I need to do a pharmacokinetic study on the blood sample collected. Which will be a better blood tube for blood collection for pharmacokinetic study? Using EDTA or heparin blood tube?

21 July 2016 1,950 3 View

Which blood tube should we use for blood collection for pharmacokinetic study?

I am currently doing an in vivo pharmacokinetic study. I will be collecting blood samples via cardiac puncture. I would like to know which type of blood tube should we use to collect the blood....

01 July 2016 3,893 3 View

Can anyone explain how to quantify gfap fluorescent intensity using image J?

I have stain my retinal slides with GFAP. I am trying to quantify the intensity of the gfap signal using image j. Currently the only method I able to find is calculating using corrected total...

14 April 2015 6,698 8 View