Which is better use: evaporative light-scattering detection or refractive index detection in sugar analysis by HPLC?

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What suitable analytical method do you suggest for the analysis of famotidine?

what suitable analytical method for analysis of famotidine?

10 November 2015 8,825 5 View

What is the new in organic chemistry?

what are the new topics in organic chemistry?

08 September 2015 682 3 View

I need a simple way to measure carbon monoxide in the air

I need a simple way to easure carbon monoxide in the air

09 October 2014 8,397 1 View

Why does formic acid give bad separation when used to improve the separation in separation sugars?

What is the effect of the formic acid in this case?

03 April 2014 5,142 4 View

What does ultrasonic assisted exraction mean?

03 April 2014 9,150 6 View

When comparing between methanol and acetonitrile to separate sugars why did acetonitrile give the best separation?

Comparing between methanol and acetonitrile to separate sugars by LC/MS we got a better signal using acetonitrile. NH2 column methanol:water acetonitrile:water

03 April 2014 9,833 4 View

What does universal bulk property detector in chromatography mean?

Is there another kind?

03 April 2014 5,856 3 View

Why does a guard column improve separation?

When I use guard column it improves separation

03 April 2014 3,217 5 View

Why can't reversed phase columns be used for underivatized sugars?

see above

03 April 2014 5,186 5 View

Why is a buffer used in ion exchange?

See above

03 April 2014 7,964 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

How to visualize stress in a coating defined by "Skin" in Abaqus?

Hello All! I'm trying to model a contact between a coated substrate and a tip in Abaqus. The coating is ultra-thin compared to the geometry of the substrate. So I defined the coating using the...

16 February 2021 1,594 3 View