Which is better for hMSC culture? alphaMEM with nucleoside or without nucleoside?

More Chi Woo Yoon's questions See All

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I use different force field for protein and ligand?

Hello, Dear! Generally, I used CHARMM36 + cgenff force field for simulaiton. But,, due to certain parts of lignad, force field cannot be generated using Cgenff. Can I use different force field...

10 July 2024 9,513 3 View

Ways to mitigate stimulation electrodes coming off?

I have a cathode electrode (5 x 5cm) on the femoral triangle and an anode electrode (5 x 10cm) on the gluteal fold. We are doing repeated high-intensity cycling followed by repeated Wingates. We...

07 July 2024 833 1 View

Is the polymer has low CTE if the polymer has the high chain packing and small free volume?

I wonder the tendency of coefficient of thermal expansion (CTE) of polymer which has different chain structure. In my opinion, CTE will be higher when the polymer materials has high interaction...

30 June 2024 570 5 View

How to solve Gaussian Convergence failure?

To whom it may concern, I'm working on optimizing the molecule using gaussian 16. However, it is suffering from the problem of convergence failure. I attempted optimization several times, but...

30 May 2024 6,373 3 View

Setting chlorine injection concentration??

I want to experiment with microbial control through chlorine disinfection. What I have on hand is 5% sodium hypochlorite solution. I want to set the chlorine injection concentration to 1, 3, 5,...

01 May 2024 3,741 0 View

Why hapolid cells show no growth after yeast tetra dissection?

Greetings to science community, I am operating Kluyveromyces marxianus yeast and want to generate haploid cells from diploid cells. However after 4 day since dissection, I found no growth of...

09 March 2024 8,360 0 View

What is the mechanism of ionization of transition metal?

can anyone give me advise about charge transfer transition of transition metal and rare earth element? and usually people see the FL and absorption, emission spectra of those materials in glass,...

08 March 2024 7,916 1 View

How to dissociate clumping cell?

H1781 cells are shown to tend to clump each other. H1781 is adherent cell and detached by using trypsin-EDTA. however, over-digestion using trypsin might provoke cell clumping. they seemed like...

03 March 2024 7,757 4 View

Optimal Approach for Repeated Measures Clinical Trials with Small Samples: GEE vs. Non-parametric Analysis - Expert Insights?

When undertaking clinical trials with limited sample size and repeated measures, the question arises: which is more advantageous—employing "Generalized Estimating Equations (GEE)" or opting for...

21 January 2024 1,357 3 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Daniel J. Medina

Both are fine for hMSC culture.

Jun Ma

I also want to know the answer. Chi Woo, did you find any difference?

Thanks,

Jun

Dai Chen

alphaMEM with nucleoside seems better.

Chen, Shangwu, Yushi Sato, Yasuhiko Tada, Yuma Suzuki, Ryosuke Takahashi, Masahiro Okanojo, and Katsuhiko Nakashima. 2021. “Facile Bead-to-Bead Cell-Transfer Method for Serial Subculture and Large-Scale Expansion of Human Mesenchymal Stem Cells in Bioreactors.” STEM CELLS Translational Medicine 10 (9): 1329–42. https://doi.org/10.1002/sctm.20-0501.