Hello Prabhakar,

I personally would suggest starting with ultrapure millipore water. This will help to ensure you are eliminating as many variables as possible that may be present in your water. My former colleagues and I would make our artificial cerebrospinal fluid with ultrapure millipore water and had great success with our chemoreception studies.

Thank you G. Andrew Pratt III

Dear Prabhakar,

You can gather some info in "How useful is SBF in predicting in vivo bone bioactivity?" by Kokubo
and Takadama.

The Appendix A, below the conclusions, may help you.

Best,

Thank you Ricardo Sanson Namur

Prabhakar

What is the purpose of creating simulated body fluid (SBF)? Is it for an implant to replace bone or for attachment to bone or for any analytical work? however, it is best to use ultra-pure water in simulating body fluid.

Dr.K

Thank you Navin Kothari sir.

My goal in creating simulated bodily fluid (SBF) is to conduct an In-Vitro test for an implant.

Prabhakar

For the in-vitro test, simulated body fluid is the best. It provides important information on the compatibility of implants with the body fluid especially how it reacts in a body and its lifespan in the body. You should do the literature survey on this in ACS - Biomaterials, Frontier in Bioengineering 7 Technology, or on Google search searching "in-vitro test for implant". Hope this helps.

Dr.K

Thank you Navin Kothari sir.

Prabhakar

Use ultra clean water to wash all water handling equipment and in making SBF for in vitro test and examine implant under high power optical microscope & SEM.

Dr. K

Thank you Navin Kothari sir