Is there an in Vitro Antibody Dependent Enhancement assay demonstrating IL-6 release in SARS-CoV-2 pseudotype virus co-culture?

I am looking for an in vitro Antibody/Sars-CoV-2 pseudotype or antigen co-culture experiment that can demonstrate antibody dependent enhancement using IL-6, a measure of indirect activation and...

17 August 2020 2,369 3 View

What are the explanations for asymptomatic CoV-19 infection in the pediatric age group?

1) Is the pediatric age group (

25 June 2020 4,054 2 View

Is anyone on this site doing original work regarding alternate theories of cosmology?

The standard model of cosmology is assumes an arbitrary number of antigravity energy infusions in order to counteract an arbitrarily-assumed global gravitational contraction force. What...

30 December 2019 5,811 31 View

Has anybody used phospho-antibodies for phosphorylation status of proteins/enzymes by intracellular flow cytometry?

Looking to use phospho-antibody (Ser177) for p-eNOS staining by flow cytometry in human umbilical vein endothelial cells.

10 June 2019 8,556 5 View

Is C60 soluble (ca. micromolar) in mesitylene or 1,2,4-trichlorobezene?

Is C60 soluble (ca. micromolar) in mesitylene or 1,2,4-trichlorobezene

14 May 2019 976 2 View

Looking for papers that demonstrate how Biostimulants enhance plant defense mechanism. Can Biostimulants replace fungicides?

Can Biostimulants replace fungicides in the management of septoria disease in wheat

19 February 2019 7,888 3 View

Seed without coat/testa improve or not the synchrony in germination?

Please I'm looking for some articles or opinion about it. Thanks

10 November 2018 3,038 7 View

Protocol for chromatin associated RNA isolation from frozen cells?

Does anyone know of a protocol which I can isolate chromatin associated RNAs from frozen cells. Essentially I am looking to monitor transcriptional output of RNA polymerases during a time-course...

22 October 2018 6,968 2 View

What is the proper way to transform and normalize large qPCR datasets for statistical analysis and heat map visualization?

I have two different tumor types, Tumor A and Tumor B, with 10 samples for each tumor type. I ran qPCR for a panel of 45 genes and I would like to plot the expression values in a heat map to...

09 August 2018 6,282 4 View

What is the standard method of coping with NA values in a double type variable machine learning problem where imputation is not appropriate?

I'm increasingly running across the case in machine learning problems where I have a double-type, continuous variable column with some NAs which should not be imputed by kNN or median type methods...

23 May 2018 3,958 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

How to compute the internal consistency of a scale with ordinal items?

Hi community, I am trying to determine what is the best way to calculate the internal consistency of a newly developed self-esteem scale. I am working together with two other researchers on the...

28 February 2021 1,369 5 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Mediation path analysis - Can I run two seperate models?

I am wanting to run a serial mediation model with 2 x IVs, 2 x MVs, and 3 DPs (all continuous). Is it problematic to run 2 different models for each IV, keeping the MVs and DPs the same in each...

27 February 2021 5,681 3 View