Hi Ajay

The only technic of DNA content mesurement I'm familiar with is the Hoechst stainning using flow cytometry.

I think that the answer to your question is different if u're interested in quantification of cells DNA content or cells ploidy.

Here are some links that could be interesting for you.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2967208/

https://www.piercenet.com/instructions/2162245.pdf

http://olomouc.ueb.cas.cz/book/analysis-ploidy-level

Good luck

You can choose a plant from the ~8500 listed in the Plant DNA C-values Database:

http://data.kew.org/cvalues/

You should select a species with a C-value relatively close (slightly larger) to the plant you want to analyse. Some plant nuclei are more difficult to isolate, so make a few tests to find the plant that works the best in your case.

You may find this paper useful:

DOLEŽEL, J., and J. A. N. Bartoš. 2005. Plant DNA flow cytometry and estimation of nuclear genome size. Annals of Botany 95: 99-110.

Do consider the challenges posed by the type of tissue on DNA content measurement even when they are from the same plant. also the similar tissue from different species behave differently to the sample preparation method. This can be taken care by co-chopping method. In case you are facing any difficulty then I can help you with the optimisation of the method with the species of your interest.