Want specific sequencing of genes for ESBL & MBL producing organisms
nano molecule with good surface area for attachment of antibiotic moiety or some other drug . how it delivers safely at right place.
03 April 2018 4,278 2 View
Metallo beta lactamase producing bacteria are increasing in clinical samples . true MBLs need to identify fast so an isolation of patient can be done at an earlier stage or necessary precaution...
08 September 2016 7,150 1 View
many patients are being tested for antithyroid antibody to rule out autoimmune cause. I frequently come around such patients .so is there any significant triggering factor for formation of these...
03 April 2016 7,096 3 View
mechanism of this autoimmune ds is not very clear so i want how to calculate for chances of Rhematoid ds developement in kids and grandsons or daughters.exact
02 March 2016 1,919 0 View
there is alteration in number and may be structural changes in surface of bacterial receptors/molecules when they acquire the resistance .To observe this change which microscopy will be more...
01 January 1970 524 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...
28 July 2024 6,938 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
