Stomatal density in plants, including Arabidopsis, is influenced by various genes that control stomata development, size, and spacing. Here are some essential genes associated with stomatal density:
EPF1/EPFL9: These genes produce small peptides that act as "stop" signals for stomatal development. When these genes are mutated, stomatal density increases. For instance, in Arabidopsis, mutants lacking EPF1/EPFL9 show higher stomatal density.
SPCH/MUTE/FAMA: These genes are like the "orchestra conductors" of stomatal development. They regulate the formation of stomata. Mutations in these genes lead to a decrease in stomatal density. For example, mutations in the SPCH gene in maize result in reduced stomatal density.
TMM: This gene ensures that stomata are evenly spaced. When the TMM gene is mutated, stomata can cluster together. This has been observed in the common bean (Phaseolus vulgaris), where TMM mutants exhibit clustered stomata.
ICE1: This gene is typically associated with the plant's response to cold stress, but it also plays a role in regulating stomatal density in response to temperature changes. This gene has been studied in tomato (Solanum lycopersicum) and shown to impact stomatal density.
ERECTA: This gene encodes a protein that influences cell growth and differentiation. Mutations in this gene result in an increase in stomatal density. This has been observed in the model plant Arabidopsis, where ERECTA mutants exhibit higher stomatal density.
BIG: This gene is involved in controlling cell division—mutations in BIG lead to higher stomatal density. In rice (Oryza sativa), mutations in the BIG gene result in increased stomatal density.
Out of the mentioned genes, ICE1 is the one most commonly associated with drought stress response. While ICE1 is well-known for its role in responding to cold stress, it also plays a significant part in helping plants withstand drought. ICE1 is like a switch that activates various genes involved in responding to drought.
When plants face drought conditions, ICE1 gets activated and acts as a messenger to turn on specific genes that help the plant deal with the lack of water. These genes control important processes such as closing stomata (pores on the plant surface), adjusting the plant's internal chemistry, and producing protective proteins.
Scientists have studied ICE1's role in drought response in plants like Arabidopsis, rice, wheat, and soybean. By manipulating ICE1, researchers have found that they can improve a plant's ability to tolerate drought.