Hi everyone,
I am searching for a DNA sequence for a particular gene from nucleotide database of NCBI. But it shows 2 or more sequences of one gene in same species. If it is due to duplication event, then ideally it should show only 2 sequences, one original gene and other resulting from a duplication event. However, in some species, I get around 3-4 different sequences. They are with the headings such as complete cds OR mRNA OR gene OR predicted ones OR same headings and all with different nucleotides.
Exactly which sequence should I consider? And if 2 given sequences are due to duplication event, then how do I confirm if they are real duplicated copies?
Dear Minal,
I am not sure about that, but you should see other nucleotide databases. The most popular one that is currently improving is Ensembl, that also offers a huge data (SNPs, mutations, predictors...) and a lot of species. The advantage of Ensembl is that it is constantly updating new data and only ONE entry per gene. Other databases are ENA-EBI, that is the European Nucleotide Database, and GENOME.JP from Japan. From my experience, the best one is Ensembl by far.
May be you can search in NCBI RefSeq
https://www.ncbi.nlm.nih.gov/refseq/
