08 June 2023 1 747 Report

Instant blue is a protein gel stain that suitable for mass spect procedure, but I want to replace it with another suitable stain for mass spect. what is your idea to change it?

More Fatemeh Vahdani's questions See All
How can I regenerate a SCX column modified with Sulfonate group (Thermo 054995)? The colmun has lost its Resolution and peak to valley ratio?

The column is used in Ion Exchange Chromatography of Monoclonal antibodies and the method of analysis is based on pH gradint.

13 July 2024 7,462 1 View

Does sucrose interfere with the BCA reagent in peptide concentration assays?

Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...

10 July 2024 6,830 1 View

What is the reason of stacking DNA in agarose gel well?

we electrophoresis PCR product on 0.7% agarose gel at 120V for 1 hour, our target size is 5000 bp but our DNA stack at wells and do not com in to gelldo you have any suggestion for this?

02 July 2024 5,235 0 View

Is it possible to increase the intensity of UV-vis absorption of DNA EITH A LIGAND IN the groove BINDING mode?

Is it possible to increase the intensity of UV-vis absorption in the interaction of the groove connection mode?

29 June 2024 4,867 4 View

Hello, I wanted to know if it is a necessity to perform MTT test in three time points to evaluate the cytotoxicity of a metal alloy?

I am working on a magnesium based alloy, and i wanted to evaluate the cytotoxicity of my alloy with MTT assay. I want to know the reason why most of the researchers perform the MTT assay in three...

28 June 2024 2,570 1 View

Population coverage analysis for some MHC II alleles?

Hello everyone Does anyone know how could we determine population coverage of some MHC II alleles such as HLA-DRB3*02:02, HLA-DRB5*0101, HLA-DPA1*02:01-DPB1*05:01, HLA-DRB4*01:01,...

20 June 2024 2,155 1 View

Separating Wind Wave and Swell Parameters in Wavewatch III Model?

Hello, I am using the Wavewatch III model for my research . Could anyone clarify if it's possible to extract wind wave and swell parameters separately from this model?

12 June 2024 3,269 4 View

Depletion igg from serum?

Hi everyone, I wanna get a suitable protocol for depletion of IgG and other immunoglobulins from serum, without kits or chromatography but with chemical methods like ammonium sulfate. Can anyone...

10 June 2024 9,523 2 View

How many sample of serum need to validate proteomics data ?

Hi everyone, I wanna do proteomics of monkeys serums in 2 states, healthy and patient. How many monkeys in each group I need to run proteomics and validate my data from it? Do I deplete albumin...

04 June 2024 4,091 3 View

How do I western blot of serum proteins without any unspecific binding of secondary antibody to my blot?

Hi everyone, I have some important problems with my western blot against serum proteins. I have used secondary antibody conjugated by HRP that binds in unspecific manner to other proteins as same...

03 June 2024 5,993 8 View

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Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

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GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

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