Hi, I am using promega master mix without dye so that equal concentration of PCR product can be loaded to DGGE. Now i did not understand which dye can be used before loading the sample. Can i used Gel Loading Dye, Blue (6X) from BioLabs? Please tell me the dye, what concentration and quantity should be used for loading?
Hi. Yes you can use Gel loading dye, Blue (6x). Bromophenol blue is the standard dye for DGGE.
0.05 g bromophenol blue in 10 ml 1* TAE
Loading Dye
0.25% bromophenol blue
0.25% xylene cyanol FF
15% Ficoll-isopaque (6.1%-18.6%)
and Ethidium bromide10 mg/ml (store in dark bottle)
Go for a 2x red dye PCR master mix (Ampliqon or Thermo). It saves time, cost effective and you could load more PCR product to the wells (this would help if the conc. of your PCR products are less).
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