H. pylori. is a fastidious organism capable of growing only under a narrow set of conditions. It must be cultured on nutrient-rich agar with whole blood from any one of a variety of large mammals such as horse or sheep. That asked and presented research input is to find out the oleaginous capacity of H. pylori. So subculture colonies are enough to carry out this research and must have followed safety label 2 during isolation and condign isolates. Now research initials in described form refers to determine oil capacity range in H. pylori either it is cholesterol or not. First reported data should be positive when the oleaginous stained is resulted as it oleaginous, than next step is to be followed for determining nature of oil that either it contains saturated or unsaturated fatty acid. Alignment of results are to be followed and screened to find out correlation of this oleaginous activity on related with producing LDL or HDL. After extraction and determination of oil in nature, the methodology will be very easier to screen out for type of cholesterol by biochemical (Both organic and inorganic) test. Best of luck brother, this is my suggestions that i learned. Thank you.