We need such a protocol for the same purpose, i.e. preparation of single-cell suspensions prior to FACS.
Dear experts, I am planning to generate a large numbers of Illumina libraries in an ongoing project. These will be amplified via a single PCR step, in which the indexed adapters are added to the...
12 May 2024 8,656 1 View
Most SD research seems to happen in adjacent fields such as economics or geography. I struggle to find papers that make use of this method for core topics of sociology. There seems to be more...
29 January 2024 2,171 1 View
I suppose, first and foremost, that the total physical number of the organisations to be studied should be ascertained. If the total number is 30 and above which is statistically large, a sample...
01 August 2023 8,596 3 View
How can I unde the accidentally deletion of entries in bionumerics ? Is there an undo function ?
20 July 2023 9,770 0 View
I'm trying to manufacture microfluidics into 75x25x1mm COP polymer slides. The process is 11 min long and using 150°C (COP Tg=100°C) temperature around 20-30bar pressure. Problem is during the...
09 July 2023 4,908 3 View
Michael Slovin in 1960 (cited in Guilford & Frucher, 1973) pioneered the sample size determination formula thus: N/1+Ne2. He received no credit for this pioneering efforts. Seven years later...
19 April 2023 8,185 9 View
For my work i did some binomial GLMs, where i compared locations with the presence of a species (wolves) against pseudo absence. Now i wanted to evaluate my Models by plotting the residuals of...
10 November 2022 6,486 3 View
I created Buffers (shapefile) around several Points of Interest. Now i want to calculate how much of these Buffers-Areas overlap with other Polygon-Shapefiles (e.g. Protected Areas, Military...
09 October 2022 4,273 4 View
Hi! I am stumbling over a weird thing which PyMol shows me in some structures: The attached image contains one of them. Between two residues from different chains, I'm getting a solid line, like a...
04 September 2022 5,116 2 View
For my thesis I'm trying to find out what influences people in their intention to use medical video consultations. I'm using an adopted UTAUT2-based model. With the first 100 responds I did a...
23 May 2022 3,643 7 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!
08 August 2024 6,242 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Visual Studio Code (VS Code) has become a popular choice among developers for several reasons: 1. **Free and Open Source**: VS Code is free to use and open source, making it accessible to...
07 August 2024 7,013 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I received an e-mail invitation to join the editorial board of Clinical Cardiology Updates. While I have published a few articles related to cardiovascular disease, there are lots of colleagues...
06 August 2024 8,981 8 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View