02 February 2019 1 8K Report

I am trying to extract a small (

More Kanomi Sasaki-Capela's questions See All
Why Are My DLD-1 Cells Still Attached to the Cloning Discs?

Hello, I am trying to isolate individual colonies using Fisher paper cloning discs. I soak the discs in Trypsin, place on top of colony, and the cells easily attach to the paper. When I transfer...

10 November 2018 4,477 0 View

Zero Blunt Cloning - Where do these bands come from???

After PCR using Q5 pol, I ligate the insert into pCR-Blunt using T4 ligase. Transformation into DH10 is successful and a few colonies are mini-prepped. The plasmid is then cut with EcoR1-HF and...

10 January 2018 2,584 8 View

Zero Blunt Cloning - Where do these bands come from???

After PCR using Q5 pol, I ligate the insert into pCR-Blunt using T4 ligase. Transformation into DH10 is successful and a few colonies are mini-prepped. The plasmid is then cut with EcoR1-HF and...

31 December 2017 6,798 9 View

If a protein is easily aggregated during dialysis in HBS, does that infer that the protein would easily crystallize?

I am working with a protein and had to dialyze the solution to remove salt.  Even though, during purification and AcTEV digestion, the proten did not aggregate, during dialysis, it obviously...

09 October 2016 3,637 7 View

Why are there no bands in my IP lanes?

I performed an IP to pull-down an endogenous protein A.  When I immunoblotted with anti-protein A, the film only showed bands for the input lanes.  The first chemiluminescence treatment was with...

07 August 2016 9,524 7 View

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How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

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Palmitic acid presence in aqueous fraction

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Which solvent is better to dissolve with secondary metabolites extracted from fungi?

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For systematic review data extraction, should I use ITT or PP analysis, and count all randomized participants or only those who completed the study?

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For the moringa oleifera extract using ethanol as the solvent,what is the alternative method to concentrate the extract other than rotary evaporation?

99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified

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What are the factors that causes the sample to have higher efficacy at lower concentration?

We conducted an antibacterial study of a plant extract. Varying concentrations of crude extract were subjected to microbroth dilution assay. The result showed that only the lowest concentration of...

31 July 2024 8,666 3 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

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How can I extract my bibliography from researchgate ?

how can I extract my bibliography from researchgate ?

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How can biogenic synthesis techniques be applied to develop plants capable of extracting and processing heavy metals from contaminated soil, and wha?

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