Kanomi Sasaki-Capela

3 Questions 7 Answers 0 Followers

Questions related from Kanomi Sasaki-Capela

Which Do You Prefer: Kit or Freeze/Squeeze?

I am trying to extract a small (

02 February 2019 8,392 1 View

Why Are My DLD-1 Cells Still Attached to the Cloning Discs?

Hello, I am trying to isolate individual colonies using Fisher paper cloning discs. I soak the discs in Trypsin, place on top of colony, and the cells easily attach to the paper. When I transfer...

11 November 2018 4,446 0 View

Zero Blunt Cloning - Where do these bands come from???

After PCR using Q5 pol, I ligate the insert into pCR-Blunt using T4 ligase. Transformation into DH10 is successful and a few colonies are mini-prepped. The plasmid is then cut with EcoR1-HF and...

01 January 2018 6,765 9 View