Hi,
I've been reading recommendations of which voltage to use for agarose gel electrophoresis and they are often given in V/cm, where the cm refers to the "distance between the anode and cathode" of the electrophoresis system. I am unsure in which direction the distance is intended to be measured?:
- diagonally across the gel chamber between the anode and cathode?
- only in the direction of the "Y axis" of the chamber between the anode and cathode? (like the direction that the DNA migrates) or
- only in the direction of the "X axis" of the chamber between the anode and cathode?
Thanks in advance.
Elin
Measure directly between the electrode wires at each end of the tank where they enter the buffer at each end of the gel tank. If you have a gel 10cm long then the distance in most gel tanks will be about 16cm between the electrodes in a mini tank
Hello,
You must measure the distance between the anode and cathode, from wire to wire, (the length of the tank), and then, multiply the distance with the recommended voltage/cm, regularly 5 volt/cm.
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