Hi! Does anyone have a method description to determine gross energy in (human) milk by bomb calorimetry? Will I need to freeze dry the samples first?
The milk sample (like any other liquid) should be encapsulated in a plastic of previously measured heat of combustion.
I am purifying different proteins that go into inclusion bodies when expressed in E. coli. When I purify my inclusion bodies I always find two bands around 37 kDa in my solubilized fraction. These...
06 May 2024 6,324 2 View
Hello there. I was looking for a simple way to get all chromosome 21's genes with their size and their protein size (amino acid number). also It's all functional and non-functional mRNAs. Is...
04 February 2023 8,808 6 View
Hi, I've been reading recommendations of which voltage to use for agarose gel electrophoresis and they are often given in V/cm, where the cm refers to the "distance between the anode and cathode"...
14 February 2021 6,748 3 View
I'm looking for information on Parvicardium hauniense and where I can find any papers, any help appreciated.
16 March 2016 133 3 View
There seem to be many different protocols for DC differentiation which makes me a little confused. I isolated PBMCs from human blood and sort out the CD14+ monocytes with negative selection using...
05 August 2012 6,528 15 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
Hello everyone, I am researching ways to enhance the quality of lamb milk replacer for machine feeding. I would appreciate any insights or recommendations on the following: Manual vs. Machine...
06 August 2024 6,227 2 View
We assume this to be true. We also assume that the vacuum bomb is the latest version of explosives with an explosive power of a few to ten kg of TNT equivalent. It has the unique characteristic of...
04 August 2024 4,534 1 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...
02 August 2024 6,773 12 View
Hi researchers! I'm working on soil texture analysis, and the end result for sand is doubtful because there is black sediment appearing after drying, as shown in the figure. Is it considered sand?...
30 July 2024 557 2 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I am doing research on wear mechanism of the additive manufactured metal parts under dry condition. Please share the reference to start the analytical modelling of the wear prediction.
29 July 2024 6,115 0 View
Since 2016 Brexit, the world needed to change the thinking behind traditional democracy as the democratic landscape changed, yet traditional democratic thinkers and actors have been acting as if...
28 July 2024 6,515 1 View
Perfect democracy thinking assumes no chaos so no need for independent rule of law system and liberal democracies assume the possibility of normal democratic chaos that can be sorted out by an...
28 July 2024 473 1 View