I have exopolysaccharide (EPS) extracted from Bacillus sp. and wanted to dialyze it overnight. From previous papers they suggested 8,000 - 14,000 Da, 12,000-14,000 Da and 3,500-4,000 Da Molecular Weight Cut Off (MWCO). I was wondering which MWCO was suitable for my bacterial EPS dialysis. How do I know that? Thank you in advance for your advice.
Poorna Manasa Bhamidimarri
Since the molecuar size range for EPS from Bacillus starts as low as 1kDa. Maybe you can use a membrane having MWCO 500-1000 Da) to avoid loss of low molecular weight EPS during dialysis.
Hope this helps.
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