Linear vs Supercoiled vs Nicked DNA on agarose?

I am pretty new to molecular biology techniques and just when I think I have it all figured out, something stumps me. This is how this gel (1.5% agarose) is loaded: 1. GeneRuler ready to use cat#...

06 October 2020 7,523 4 View

Has anyone reported on carbon storage potential in common trees and shrubs in prairie forests?

Has anyone reported on carbon storage potential in common trees and shrubs in prairie forests? I am looking for carbon storage potential ie carbon biomass for a number of native trees and shrubs...

08 July 2020 5,236 2 View

Issues with Image J Thresholding?

I am wondering if anyone has had issues with their image J thresholding? I am trying to perform colour deconvolution, but when I convert the image to binary, I am unable adjust the threshold to...

01 April 2020 3,812 3 View

Where can I get survey data to compare the US to subSaharan Africa, China on the Impact of Climate Change?

Preparing a research paper. Other countries/geographies could be substituted

12 March 2020 195 4 View

Quantifying ADC values from a ROI?

I currently have both DWI and ADC sequences however I want to calculate the mean ADC value for a defined ROI (ischemic stroke lesion). I currently use ITK-SNAP, Image J and Horos for viewing...

03 March 2020 9,749 4 View

Which software is good for analysis of band size in gel image? How can we analyze the curve band image?

Currently, i am thinking about GelAnalyzer software. Please help me with the analysis and band scoring. I have used 100 bp ladder.

17 February 2020 7,762 5 View

Can i be updated on conferences for Graduate Students in the field of Clean and Renewable Energy?

06 August 2019 2,648 3 View

How do I align a sequence set to one reference sequence?

I am currently trying to find homologues of a protein I am working with, but BLAST has been giving me nothing useable. I have now found a dataset of 1500 protein sequences of potential candidates...

26 June 2019 5,596 2 View

I need an analytical balance that weighs organic samples at 0.0001 g for stable isotope analysis. What models are reliable and cost-effective?

For weighing stable isotope samples

18 October 2018 687 4 View

Assuming you are teaching a class and a student ask you a question you cannot readily answer. What would you do?

When you find yourself in this situation, what would you do intelligently so your students don't believe you can't answer?

02 March 2018 818 62 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View