I have used the Quant Studio 3 instrument and the Taq Man Genotyping Master Mix from Applied Biosystems for a genotyping PCR reaction, but I got any signal, even not of the reference dye (ROX). After the PCR run the tubes were ok with the expected volume (11 uL), without bubbles, droplets or condensations. What could be the reason of this result?
The master mix was new but I have to add that it were accidentally stored at -20 ºC for a period of a month and the instructions were storing at 2 to 8ºC, could be that this has been spoiled?
Greetings
are you sure the instrument is compatible with the TaqMan?
It's very possible that the reagents were degraded by improper storage. One heck of an expensive lesson to learn, but that's my guess.
Buy a new reaction mix, store it as indicated by the company, and try again.
Good luck!
