I'm trying to detect surface GluA1 levels in dissociated neurons both at steady state and after a chemical LTP paradigm.
It seems like many labs use a Millipore antibody (MAB2263/RH95) on PFA-fixed non-permeabilized cells (although there should be concerns about PFA-mediated permeabilization).
I have also tried PC246 from Millipore in a live-labeling approach without good results.
Any other antibodies working well for others?