Andrew Coleman

4 Questions 7 Answers 0 Followers

Questions related from Andrew Coleman

Why a lack of Cre-mediated recombination following nucleofection of post-natal mouse cortical neurons?

Hi all, I am trying to delete a protein of interest from postnatal cortical neuron cultures from floxed mice by introducting Cre via nucleofection (AMAXA system), but fail to see any loss of...

10 September 2018 7,327 2 View

Which commercially available N-terminal GluA1 antibodies work best for surface staining in dissociated neurons?

I'm trying to detect surface GluA1 levels in dissociated neurons both at steady state and after a chemical LTP paradigm. It seems like many labs use a Millipore antibody (MAB2263/RH95) on...

08 December 2017 1,518 0 View

Can you help me identify this mouse subcortical brain region?

I am interested in identifying the brain region displaying relatively high immuno-reactivity for a protein of interest (red) in the attached images of a mouse brain (co-labeled for NeuN-green). I...

07 October 2015 7,942 7 View

What MOI should I use for infecting primary mouse cortical neuronal cultures (P0) with AAV (serotype DJ)?

I would like to infect a high proportion of mouse cortical neuronal cultures from P0 pups with AAV for a biochemical study while avoiding toxicity. I would like some suggestions on the MOI (ratio...

06 September 2015 7,541 2 View