Andrew Coleman

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Questions related from Andrew Coleman

Why a lack of Cre-mediated recombination following nucleofection of post-natal mouse cortical neurons?

Hi all, I am trying to delete a protein of interest from postnatal cortical neuron cultures from floxed mice by introducting Cre via nucleofection (AMAXA system), but fail to see any loss of...

10 September 2018 7,262 2 View

Which commercially available N-terminal GluA1 antibodies work best for surface staining in dissociated neurons?

I'm trying to detect surface GluA1 levels in dissociated neurons both at steady state and after a chemical LTP paradigm. It seems like many labs use a Millipore antibody (MAB2263/RH95) on...

08 December 2017 1,476 0 View

What MOI should I use for infecting primary mouse cortical neuronal cultures (P0) with AAV (serotype DJ)?

I would like to infect a high proportion of mouse cortical neuronal cultures from P0 pups with AAV for a biochemical study while avoiding toxicity. I would like some suggestions on the MOI (ratio...

06 September 2015 7,505 2 View