Dear ResearchGate community,

I am currently conducting research on the role of human alpha-klotho in blood and cerebrospinal fluid (CSF), and I am looking for recommendations on the most suitable ELISA kit to use for measuring alpha-klotho levels in these matrices. I am aware that there are several commercially available kits, but I am interested in hearing from other researchers who have used these kits and their experiences. Specifically, I would appreciate suggestions on which kit has been the most reliable, sensitive, and specific in detecting human alpha-klotho in blood and CSF samples. Additionally, I would like to know which factors should be considered when choosing an ELISA kit for this purpose, such as the type of sample matrix, cross-reactivity with other proteins or substances, and assay format. Any insights or experiences related to measuring alpha-klotho levels in CSF samples would be especially valuable.

Thank you in advance for your contributions and advice!