Phenomenex has kindly loaned me a Rezek (RCU) column for oligos (raffinose, verbascose etc) analysis. I started by using only water as mobile phase and will try introduce acetonitrile to set up the method. What other columns will work for these compounds and which mobile phase system is recommended.
Your suggestions are really appreciated.
We do oligosaccharide analysis with a normal C18 reverse phase with only water with an RI detector at 1.5 to 2 mL /min. flow. Regards,
Hi Jorge, thanks for the input. Are you looking at raffinose, verbascose and stachyose too? any tips for sample prep for your method? thanks
No, not that sugars, we use the method in following starch hydrolysis and in corn syrups control. The injected sample is only diluted and filtered trough 0.45 micro . regards,
Simple sugar is easier to detect than oligos. Thanks anyway
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