Which column and solvent gradient can give the best spectra of ESI-MS for Aldolase in LCMS ?

More Neelesh Reddy's questions See All

Dear all, Iam currently working on Dc Microgrid model for electrical vehicle charging. Can any body give good IEEE research paper on this topic?

please give any suggestion?

04 February 2021 4,837 5 View

What does the change in D-peak position in Raman spectroscopy of carbon materials indicates?

We have carried out high-pressure Raman characterization on some of our samples. After fitting, I have observed a change in D-peak position with increasing pressure. What does this change...

20 January 2021 853 3 View

PSFB control signal?

Hi everyone iam facing a problem to give control signal for switches in design of PSFB converter in matlab simulink. Anybody's help will be appreciated.

14 December 2020 6,021 3 View

What are the alternative to the pozzolanic materials to in concrete to enhance the durability of concrete?

As of now, Only industrial wastes were using as pozzolanic materials in concrete to enhance the durability of concrete. Can any once suggest me the alternatives of those materials?

11 October 2020 3,569 4 View

Can any one suggest me the dielectric constant of Gold and Co3O4 at room temperature ?

i have a question regarding dielectric constant of gold,cobalt oxide (co3O4) and n-tpe silicon . i find only one publication on this issue showing dielectric function cobalt oxide(CoO) 12.9 and...

02 October 2020 9,138 4 View

Eupergit beads for enzyme immobilization?

We are looking to immobilize some of our enzymes on Eupergit beads. I was wondering if you know any commercial supplier of Eupergit beads?

28 September 2020 5,664 3 View

Can somebody kindly suggest a good FEM software for the numerical solution of stochastic PDEs?

Please kindly suggest some good FEM softwares for the numerical solution of both time-dependent and time-independent stochastic PDEs.

28 September 2020 6,598 4 View

How to run Seismic analysis for the following model in OpenSees..?

I have developed a 3 bay 5 story 2D frame in Opensees but I have problems with element load and application of seismic load in Opensees using an acceleration file. I always got an error namely...

27 September 2020 5,885 4 View

How can we evaluate the conductance of a constant power load (CPL)?

Any CPL is modeled with a current source in parallel with negative resistance. How do we evaluate the negative resistance, if we know the constant power consumed by the load?

19 September 2020 6,188 3 View

Calculation of effective mass using wannierised band tructure.?

Method to calculate, effective mass of any 2D material of Graphene family using Wannierised Band structure with HSE06 functional at Dirac point (linear dispersion).

10 September 2020 9,356 1 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

Eef Dirksen

Hi,

For that type of analysis, I have good experience with Agilent's PLRP-S columns (1000A), ran with a water/acetonitrile gradient (with some formic acid) at elevated temperature (70 degrees Celsius).

Good luck!

Neelesh Reddy

Dear Eef,

Can you suggest something which can be tried at room temperature?

best regards,

Neelesh.

Yes, then I guess I'd try HP-SEC coupled to MS. I'm not sure if you worried about protein denaturation at elevated temperatures; the organic solvent will induce that too.

Depending on your question/goal, you could try to include acetonitrile in the SEC running buffer (together with some volatile salts, such as ammonium acetate) or got for a native approach, with volatile salts only.