Some anti-viral drugs are inactive prior to phosphorylation, but they become active after their phosphorylation within the cells. Which other class of compounds become phosphorylated inside the cells? Are these phosphorylated compounds detectable with HPLC, HPLC-MS and GC-MS?
As far as I know just the nucleoside(-analog)-compounds get phosphorylated, since they are resembling RNA/DNA-building blocks.
Besides the antivirales (best example is aciclovir) also some cytostatic agents used in cancer diseases get phosphorylated by the same mechanism. A example for those compounds is azidothymidine (zidovudine).
Since all of those compounds have a aromatic nucleobase, they should be detectable by HPLC, also the phosphate makes them way more hydrophilic so the retention time is different.
Vitamin B6, pyridoxal and pyridoxine, is phosporylated within cells bij pyridoxal kinase. Pyridoxal phosphate acts as a cofactor. The B6 vitamers can be quantified by LC-MSMS
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