I'm planning to start designing an in vivo assay to be used for a HTS. Does anyone have any advice/know of literature on the subject on how to choose an appropriate cells line? I will have to establish a stably transfected cells line with at least two constructs. The readout will be measuring changes in GFP expression.
Dear Mrs. Bobrowska, I believe there is no right recipe for that, but it will really depend on the endpoint or biomarker that you are interested in. In your case, as you are transfecting a cell line with two constructs, I would suggest you to look for references on transfection rather than on HTS. I will try to contact some people that work with transfection to check if I can assist you any further.
Best regards,
Patricia
The attached article could be of help ... but maybe too late ...
Best regards
Robert
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology
- Culture Cells
- Cell Line