Hello, Anna,

I use the MMI Cell Cut Plus system and perhaps the 0.5 ml tube fits into your system: (http://www.molecular-machines.com/products/consumables/mmi_isolationcaps)

0.5 ml, 50 pieces (Prod. No. 50202)

There is information about local distributors in their website.

Best wishes,

Vanessa Bernardes

Thanks Vanessa, this is very helpful.

Dear Anna,

another possibility would be to follow the LEICA manual for the Laser dissection system: cf. p-13-, Version 1.0, February 2012

(e.g. http://www.unige.ch/medecine/bioimaging/equipment/microscopes/protocoles.pdf )

Microcentrifuge Tubes for Specimen Collection

During LMD, the specimen falls via gravity into the cap of the selected microcentrifuge tube. The cap may be used dry, but can also be filled with buffer. In this way, degradation of nucleic acids can be prevented, or proteins can be directly rescued for electrophoresis or chromatography. This might be crucial when cutting out hundreds or thousands of individual cells.

Regular 0.2 ml and 0.5 ml microcentrifuge tubes are mounted into the collection devices. Please note that microcentrifuge tubes of some suppliers may not fit into the collection device because of the shape or the size of a particular cap.

Suitable tubes are supplied by Greiner Bio-One. In addition, it is possible to accommodate particular types or brands of tubes with specially fabricated tube holders. The specimen might also be collected directly into 8-well strips. This option is regularly used for the Live Cell Cutting (LCC, see page 32). Compatible 8-well strips are from Greiner Bio-One (e.g. 762071).

For more detailed information on collection devices, please refer to the Instruction

Manual or contact your local Leica Microsystems representative.

Greiner PCR Tubes

Order no. Leica Microsystems Size Order no. Greiner

11 090 615 027 006 0.5 ml 682 201

11 090 615 028 006 0.2 ml 683 201

GREINER: for US see

http://us.gbo.com/bioscience/inter_brochure, for PCR-Tubes 0.5 ml:

http://viewer.zmags.com/publication/84b0fcea#/84b0fcea/138

Best wishes, good luck and regards

Wolfgang MUSS, SALZBURG, Austria

(PS: It might be possible that there are other dealers offering the same or similar PCR-Tubes. No financial interst, no affiliation with GREINER.com)

hi, we also have an LMD6500 - we use the Greiner thin wall tubes, as specified by Leica (Cat# 682 201)

josef

@Vanessa and others:

Dear Vanessa, just FYI (for your next acknowledged posting (:-)) )

if you insert an URL please be sure to separate the URL itself from the "parentheses" (),

e.g: not:

(http://www.molecular-machines.com/products/consumables/mmi_isolationcaps)

this URL will turn out be , but write / use instead:

( http://www.molecular-machines.com/products/consumables/mmi_isolationcaps ) or best: don't use parentheses. Thank you,

best wishes and regards,

Wolfgang

WE use Thermo Scientific PCR Tube, flat cap 0.5mL 1000/cs, #AB0350

Thanks to everyone for help. I will try the recommended tubes. I'm concerned that our tube holder is somehow more restrictive than the ones I see on Leica website. For those who recommended Greiner and Thermoscientific, do you have a holder that will only fit a specific cap width (i.e. 0.78 cm - only as wide as the tube mouth)?

I have used Carl Zeiss microtubes in LMD6000.