Hello, everyone. I appreciate if anyone can answer my question. i want to analyze the cytotoxic ability of CD4+ T cells subset in vitro by CFSE assay. I need to know the appropriate cell line for this assay. k562, Duaidi, and NIH-3T3 ???
Dear Dr. Yashgin Hassanzadeh!
Here are the requested data about cell line and proper estimation of cytotoxicity!
All the best!
Sincerely Yours!
Dr. Bratko Filipič
E-mail: [email protected]
P.S.: Hope you will inform me about the success!
Thank you, dear Dr. Bratko Filipic. your articles didn't have cell line proper for CFSE cytotoxicity assay. But i appreciate your kindness.
Hi Yasgin,
At my knowledge,
CD4 is expressed by Monocytes and macrophages, I have used U937 (human monocytes) and NR8383 (rat macrophages) cell lines before (for another purpose). Both cultures are easy and work very well.
Good luck.
Nina.
Dear Nina, thanks for your answer. CD4+ is a key marker for T helper cells (lymphocyte).
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