Which buffer and method is suitable for extraction of proteins from animal tissue?

There are several steps you need to consider and before you decide on a method you need to know your downstream application as not all reagents are compatible with mass spec or proteomic methods for example. Here are a few resources but by no means an exhaustive list, there is lots of info on the net, but this will help you get started. Several suppliers have useful resources on their websites, but you can still shop around for the product from other suppliers. This paper has a good background on sample preparation approaches as well as a general into to protein analysis methods.

http://www.sciencedirect.com/science/article/pii/S1570023206008555

You need to also be careful to prevent protease and possibly phosphatase activity. There are many commercially available cocktails for this purpose, again the suitability of these depends on your downstream application. The Thermo website has a nice table listing common inhibitors and their features, and the labome website has a nice summary on protease inhibitors in general.

http://www.labome.com/method/Protease-Inhibitors.html

http://www.piercenet.com/method/protease-phosphatase-inhibitors

Finally quantification of your protein yield can be achieved by several methods but make sure you use one compatible with the reagents you have selected for protein prep. Again Thermo/Fisher/Peirce/Invitrogen (I lose touch with who is with whom) have a nice table showing compatibility of protein assays which is now in this handbook.

http://www.lifetechnologies.com/nz/en/home/life-science/protein-biology/protein-assays-analysis/protein-assays.html

As you can see there isn’t a simple answer to your question, but I hope this all helps you start investigating the correct method for you. Remember to keep everything cold all through the process of protein extraction and prevent freeze thaw cycles in your final samples as this will degrade and alter them fast.

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