Which bacteria can also grow in PALCAM agar other than listeria?

More Dipika Malakar's questions See All

Which animal model (rat or mice ) will be appropriate for study of wound healing?

I am to study the wound healing of infected wound in animal model. However I am not sure about the animal model to be used. Whether swiss albino mice or Wister albino rats or Sprague Dawley rats...

26 February 2020 2,565 2 View

What is the maximum rpm and minimum temperature that would facilitate biofilm formation invitro?

I have been trying to obtain the biofilm of Staphylococcus aureus on glass coverslip to visualise it under the microscope, however no fruitful result has been so far obtained.

19 December 2018 787 4 View

What is the purpose of using stain and destain in SDS-SDS-PAGE gel?

Purpose of using stain and destain in sds page gel

15 March 2017 4,107 9 View

How can I prepare deactivated silica gel with 12%v/w water for chromatography?

The mobile phase is EtOAc-hexane (2:8)

10 October 2016 9,344 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

Dipika Malakar

Thank you Sir for the information.

Abdelraouf A. Elmanama

Several M.Os can grow on PALCAM which is selective for listeria. Partial inhibition of others bacteria does not guarantee absolute inhibition. E coli, Pseudomonas and S. aureus can possibly grow especially if they are in high concentration in the sample .

You can check this link for further details

best wishes

http://www.bd.com/europe/regulatory/Assets/IFU/HB/CE/PA/PA-254539.pdf