What is the maximum rpm and minimum temperature that would facilitate biofilm formation invitro?

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How to filter structural variants from WGS/WES data?

Hello everyone! I would appreciate some advice regarding the filtering of structural variants. The variant caller used for discovery-“DELLY or DELLY2”using whole-genome sequencing and Whole...

09 July 2024 498 1 View

DNSA assay for alpha amylase inhibitory activity shows unexpected trends in sample extracts. Seeking guidance for improvement?

I have been conducting an alpha-amylase inhibitory activity assay using the DNSA (3,5-di-nitrosalicylic acid) method with Acarbose as the standard inhibitor. The absorbance values obtained for...

18 March 2024 1,411 3 View

How does He gas leak in ICP-MS effect the sensitivity of Ir and Rh causing their RSDs go higher?

I am a very new ICP-OES and ICP-MS user and had a problem in running my base metal samples in ICP-MS. For some reason which I don't know the calibration and analytical results are full of poor...

24 November 2023 1,307 1 View

Does anyone has 'Yale Face' and 'AR' dataset?

Hello, I am writing a paper on face recognition. I have LFW and CASIA-WebFace datasets. but I need two more datasets. If anyone has 'Yale Face' and 'AR' datasets please feel free to give them to...

07 February 2023 7,666 4 View

How to create nitrogen atmosphere in small scale lab during formulation?

I am an M.pharm student working on project regarding keratin alginate nanogels where i have to maintain nitrogen atmosphere during the formulation process. Can anybody tell me how do i obtain and...

28 November 2022 5,161 4 View

How can we apply Foam finishing on Knitted Fabrics in conventional textile industry and what factors can affect the power consumption ?

Foam finishing is a method that uses air as a dispersing medium to disperse finishing chemicals as a foam and that foam is speeded evenly on the fabric surface, it saves water and energy required...

12 October 2022 623 2 View

What should be the lower limit of cell viability to consider whether a sample is cyto toxic or not?

While evaluating the cytotoxicity of a sample in cell line, what percentage of cell viability is considered inorder to conclude that the sample is toxic. Like if 80% of cell survives, is the...

04 September 2021 8,898 4 View

I am having trouble growing cells?

Hello, I have been growing both adherent and suspension cell cultures from 2 years. Recently a problem has occurred. Cells started developing background, not bacterial or fungal since the media...

03 October 2020 6,026 3 View

What kind of optimization is done through genetic algorithm in architecture?

Difficulties and types of optimization

11 May 2020 6,336 1 View

Which animal model (rat or mice ) will be appropriate for study of wound healing?

I am to study the wound healing of infected wound in animal model. However I am not sure about the animal model to be used. Whether swiss albino mice or Wister albino rats or Sprague Dawley rats...

26 February 2020 2,846 2 View

Fabrication of narrow glass capillary (inner diameter 200~500 nm)?

I need a very narrow glass capillary for my research. How can I make a glass capillary with an inner diameter of around 200 to 500 nanometers?

08 August 2024 8,915 0 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

What are the best practices for washing and preparing fruits and vegetables to reduce pesticide residues?

27 July 2024 9,147 4 View

Does anyone know a source for theta-replicating Staphylococcus plasmids that are compatible?

I am trying to put 3 separate plasmids into Staphylococcus aureus and I am looking for those that are theta replicating and compatible. I have obtained pSK9065 (from pSK1) from Addgene, but the...

23 July 2024 6,968 0 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

Does the potentiostat have to be in a fume hood when polymerising the PANI?

I will polymerise Aniline on ITO glass WE, using 1M of H2SO4 and a potentiostat. However, I am not sure if I have to do the electrochemical polymerisation inside a hood. Is it necessary? Is the...

10 July 2024 2,937 3 View

Can type 2 diabetes be reversed at any age through proper diet and exercise?

Type 2 diabetes can be managed and, in some cases, put into remission through proper diet and exercise, regardless of age. Here are some key points: Weight Management: Achieving and maintaining a...

07 July 2024 8,745 2 View

Where to source glass rings compatible with cell culture?

Hi all, I am looking glass cilinders that have an open flat bottom and top. Preferably with the inner diameter in the range of 2 cm. It's similar to the ones found on multi-electrode arrays for...

06 July 2024 5,846 1 View

DNA extraction from clinical sample of Staphylococcus aureus?

Salam everyone, So I am trying to extract DNA from isolates of clinical samples of staphylococcus aureus, I am using a QIAamp UCP kit. I followed the same manufacturer protocol and used Qubit to...

03 July 2024 3,892 1 View

What is alternative for PMMA?

If we want to introduce a material similar to polymethyl methacrylate "with the characteristics mentioned in the next paragraph" that can be added to a precursor and create a composite, which...

03 July 2024 2,371 1 View