I'm trying to select one assay to measure glucose uptake/ utilization in beta cells/islets. Do you know which one is more accurate to use, a conventional radioactive using tritium label or colorimetric kit by Abcam? I want to know if I can achieve the same result without using radioactive material. Thanks
Glucose oxidase couple chemical detection of glucose can be adequately accurate when used within the range of glucose concentration that is suitable for the method. However, use of H3-glucose can be far more sensitive as well as accurate.
For studying kinetics of glucose transport, [H3] 2-deoxyglucose (non-metabolizable) works very nicely.
Is the colorimetric kit meant for glucose uptake studies or the GOD-POD based kit for serum/plasma glucose?
For fluid analysis enzymatic spectrometric methods with an appropriate calibrator I would have thought.
I worked with Biovision Glucose assay kit (Catalog #k606-100). We used the Fluorometric assay and could detect glucose in saliva. Please see the paper: Hartman M-L, Goodson JM, Barake R, Alsmadi, O, Al-Mutawa, Ariga, J Soparkar Behbehani J, Behbehani K, Welty, F. 2014 Salivary glucose concentration exhibits threshold kinetics in normal-weight, overweight, and obese children. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy 2014:8 9-15. I did not compare this glucose test to the radioactive one therefore I don’t have an information on that.
Mor-Li,
Interesting work and a potentially promising strategy for non-invasive monitoring!
Long-term follow up should give a good idea about predictive value of measurements based on measurements from saliva samples.
An approximately 13x lower glucose levels in saliva compared to the plasma and relatively compressed range (small rise in hyperglycemia) combined with accuracy and precision of glucose determination may pose a practical limitation.
Hello! Does anyone can advise me on how can I measure glucose in macrophage culture medium? I am looking for a simple test. Could be also to test glucose uptake. Someone?
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