What to do if i am getting very light bands on SDS-PAGE ?

I didnt add any molecular marker in sample. moreover light bands are seen. My protein is showing proteolytic activity and fibrinolytic activity too. But bands are very light . please tell me...

16 August 2019 7,779 6 View

Which algorithm might be worth trying for this dataset ?

Please find the sample dataset attached. Here, as we can observe there is a forecast and actual data. However, we have only for about 8 years (2000- 2008) of data. Trying: XGBoost, ARIMA...

07 August 2019 8,765 3 View

Let's say that I have a plot of Biomass concentration v/s time. How do I determine the specific growth rate curve?

I am fairly new to the field of Biotechnology.

19 February 2018 5,936 5 View

What and where could I find research project internships(summer) at US universities. Ps: I am a second year Btech (mechanical) student.

03 July 2017 9,818 1 View

Could you please suggest me an alternative Calibration method?

-I want to calibrate an economical self-designed four-point bending setup. (Please refer to the image) -The plan is to test specimens on my setup and the standard Bending setup with specimen...

18 April 2016 1,983 10 View

What are the best markers to distinguish between pericyte and smooth muscle cells?

I am working on generating pericytes and SMCs but since both have common origin it is difficult to distinguish between the two lineage best on markers. Does anyone know of very specific markers...

17 May 2013 5,297 15 View

Can someone kindly help me with a simple laboratory procedure for Detection of viability of spores of LAB?

For my research on formulation of fermented product from vegetable agro-wastes i wish to check for the viability of spores of LAB to create a probiotic soup mix.

06 December 2011 3,987 1 View

How to interpret the linear regression coefficients ?

We are trying to understand the impact of number of workdays on sales. Please find reprex below: ``` library(tidyverse) # Work days for January from 2010 - 2018 data = data.frame(work_days =...

01 January 1970 6,614 8 View

Which regression algorithm could be applied for correcting measured values?

Please consider the sample dataset below. In simple terms, An sensor is defective and hence measurement has incorrect values since 2000 and we have the data for 10 years with both: measured and...

01 January 1970 4,812 3 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Which transition metal salt precursor is best for doping? Why?

I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...

27 February 2021 4,285 3 View

What software programs can directly simulate Additive Manufacturing Directed Energy Deposition (DED)?

I understand there are a lot of software programs that can simulate DED by going through specific modeling, simulation, and commands. For me, that was time consuming and results were not accurate....

24 February 2021 4,428 3 View

It is necessary to reflux the Schiff base and metal salts to obtain metal complex??

24 February 2021 9,166 3 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

How to build fluid structure interaction simulation for elastic ring squeeze film damper in COMSOL?

How to build fluid structure interaction simulation for elastic ring squeeze film damper in COMSOL?

22 February 2021 5,408 1 View