What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Special issue on PLS-SEM in Mathematics. Are you interested?

21 February 2021 5,601 3 View

How to calculate the TCID50 when the highest CPE is 50% or less?

I am doing TCID50 to measure progeny virus and some treatments protected the cells in a way that the highest CPE observed is 50% or less. How should I do the titre calculation in that case? Thank you!

16 February 2021 8,768 1 View

Which drug is better to modify blood pressure in awake monkey, if I provide it in the food or drink reward?

I need to modify the blood pressure in a monkey while is awake in the recording chamber, and I am planing on provide a drug for that in the food or water used for reward... any advise? Thank you

08 February 2021 9,900 1 View

Why stop the reaction in ice for the fluorescent probe FDA (fluorescein diacetate)?

Hi, In literature I noticed that who stains haemocytes with FDA for 30 min then stop the reaction in ice while this does not apply for 15 min incubation time (or maybe authors did not write this...

08 February 2021 3,366 3 View

How to make Fast Green FCF from powder?

Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...

07 February 2021 1,671 2 View

Why do high doses of humic substances inhibit the growth of corn roots? Is there a toxicity effect?

Length, volume, surface area and dry mass of corn roots treated with humic substances increased up to 13 liters per hectare and decreased with higher doses.

02 February 2021 2,190 4 View

Using a nucleosil c18 column, i get no negative peaks in real time and two negative peaks in post run.Software problem?

Simadzu, lc solutions

02 February 2021 1,969 2 View

Any ideas on fast and easy way to stain mucus on intact cell layer?

Dear RG community, We are looking for a fast and easy way to stain mucus on a cell layer that allows for macroscopic visualization of mucus distrubution. Our epithelium is grown in conventional...

31 January 2021 6,790 2 View

I.P. drug injection- time to reach brain?

I am injecting different serotonergic drugs into mice i.p. and see an effect in the brain electrophysiology quite fast, i.e. within minutes, when using a specific receptor agonist drug. I do not...

01 February 2021 8,406 3 View

Where could I purchase whole cells with 5-HT (serotonin) 1B receptors?

Hi! I would like to study 5-HT 1B (Serotonin 1B) receptors and conduct a Gi CAMP assay on it. As you know, this is a functional assay that requires whole cells and not membrane preparations. Do...

19 January 2021 3,614 2 View

What is the significance of serotonin and its metabolite on stress ?

What is the significance of increasing serotonin level of stressed rat compared to normal one and slight increase of 5HIAA level in stressed rat. With higher 5HIAA/5HT ratio in normal one.

21 October 2020 2,165 5 View

Please suggest some articles or websites about serotonin hormone?

Biological Psychology

04 October 2020 1,475 3 View

If we cannot come to actually see ourselves as a species among other similarly biological-behavioral species, can we really accomplish anything?

If we cannot come to actually see ourselves as a species among other similarly biological-behavioral species, can we really accomplish anything? I say NO -- not anything significant involving...

06 June 2020 5,833 12 View

Can sleep problems in patients with Parkinson's disease be about serotonin?

Sleep problems can be seen in patients with Parkinson's disease. Can it be about serotonin? In mice, ablation of the raphe and no production serotonin increases wakefulness and impairs the...

05 March 2020 843 3 View

Role of 5HT3 in cognition?

From the literature it was understood that 5HT3 agonist declines the memory and 5HT3 antagonists promotes cognition but i have seen the literature where it was mentioned that over expression of...

02 March 2020 3,317 3 View

Basic Psychology Research Preparation: Isn't the time/space(place) to look for specfics when one knows the time/space they are in?

Basic Psychology Research Preparation: Isn't the time/space(place) to look for specifics __when one knows the time/space they are in? It is proper contextualization (with a correct process and...

09 February 2020 9,523 4 View

Basic Psychology Research Preparation: Isn't the time/space(place) to look for specfics when one knows the time/space they are in?

Basic Psychology Research Preparation: Isn't the time/space(place) to look for specifics __when one knows the time/space they are in? It is proper contextualization (with a correct process and...

01 February 2020 9,548 3 View

Hi! I want to mark serotonin in brain tissue with an immunofluorescence. I need to know how to remove the natural autofluorescence of the tissue?

The brain tissue its from a lizard. We want to see de serotonin mark in the pineal complex.

28 January 2020 8,210 3 View